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Taq polymerase utilized in PCR Is a... | Filo
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In which technique do we use Taq polymerase enzyme and why?
In which technique do we use Taq polymerase enzyme and why?
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Taq polymerase is an enzyme that helps make new DNA strands during PCR. It comes from a heat-loving bacterium called Thermus aquaticus, so it does not get destroyed at high temperatures. During PCR, the reaction is heated up to 95°C, and Taq polymerase survives these heat cycles. Taq works best at 72°C, where it adds DNA building blocks (dNTPs) to copy the target DNA. It is commonly used in PCR because it is stable, fast, and easy to use, even though it can make small mistakes. #bioinformatics #
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